8809451.qxd - Septi-Chek .qxd
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چکیده
AND EXPLANATION Blood culture is one of the most important and critical procedures performed in the microbiology laboratory. Since blood is normally sterile, the isolation and identification of an organism has great diagnostic significance. Blood cultures are of great importance in diagnosing such conditions as endocarditis, typhoid fever, pneumonia and other diseases characterized by bacteremia. The use of a biphasic blood culture system has been shown to improve the sensitivity of blood culture over traditional broth media.1-3 When affixed to BBLTM SEPTI-CHEKTM Blood Culture Bottles after their inoculation with blood, the agar surfaces on the slide allow the subculture of aerobic, facultative and capnophilic microorganisms present in the specimen after the bottle with the slide attached is tilted and further incubated. The presence of resins in blood culture media has been shown to significantly increase the recovery of clinical pathogens from specimens.4,5 This increase in recovery is more pronounced in specimens in which antimicrobials are present, but there have also been significant increases in recovery from specimens which do not contain antimicrobials. The BBLTM SEPTI-CHEKTM TSB with Resins Culture Bottle, when used together with the BBLTM SEPTI-CHEKTM Slide, combines both features, and has been shown to be applicable for bacterial isolation in the presence and/or absence of a wide variety of antimicrobials (see “Performance Characteristics”). The presence of both nonselective and selective differential agar media on the slide allows a pre-differentiation of the microorganisms present in the liquid medium of the blood culture bottle.
منابع مشابه
Comparison of the Bactec Peds Plus pediatric blood culture vial with Roche pediatric Septi-Chek for blood cultures from pediatric patients.
Equal volumes of blood from 4,112 blood cultures were inoculated into one Bactec Peds Plus bottle and one Roche Septi-Chek bottle. There were no significant differences in the recoveries of bloodstream pathogens. Initial detection occurred earlier with Bactec Peds Plus, while growth on solid media occurred earlier with Roche Septi-Chek.
متن کاملControlled comparison of a new Becton Dickinson agar slant blood culture system with Roche Septi-Chek for the detection of bacteremia and fungemia.
Two commercially available agar slide blood culture systems, Septi-Chek (Roche Diagnostics, Div. Hoffmann-La Roche Inc., Nutley, N.J.) and VACUTAINER agar slant (Becton Dickinson [BD] Vacutainer Systems, Rutherford, N.J.), were compared in 5,628 paired blood cultures from adult patients. The systems were inoculated with equal volumes (5 ml) of blood. There was no statistically significant diffe...
متن کاملControlled clinical comparison of two lysis-based blood culture systems, isolator and Septi-Chek Release, for detection of bloodstream infections.
A controlled clinical comparison was made of the Isolator (Wampole Laboratories, Cranbury, N.J.) and the Septi-Chek Release bottle (Roche Diagnostics, Nutley, N.J.). From 6,345 blood culture sets fulfilling minimum criteria for volume of blood cultured, 840 strains were isolated, of which only 691 (82%) were considered to be representative of bloodstream infection according to Centers for Disea...
متن کاملControlled evaluation of BACTEC Plus 26 and Roche Septi-Chek aerobic blood culture bottles.
Blood culture bottles that accept up to 10 ml of blood have recently been introduced for the BACTEC nonradiometric blood culture system. The new formulation, designated BACTEC Plus, contains 25 ml of tryptic soy broth, 0.05% sodium polyanetholesulfonate, and mixed resins. In a collaborative study conducted at three university hospitals, we evaluated the BACTEC Plus 26 (BP26) aerobic bottle and ...
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